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KMID : 0380020190340020114
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Korean Journal of Biotechnology and Bioengineering
2019 Volume.34 No. 2 p.114 ~ p.119
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Anti-cancer Effect of Cinnamomum camphora Ethanol Extract by Double Induction of Apoptotic and Autophagic Cell Death in HCT 116 and HT-29 Human Colon Cancer Cell through the mTOR Signaling Pathway
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Nam Gun-He
Jo Kyung-Jo
Park Ye-Seul
Kawk Hye-Won
Wee Ji-Hyang
Kim Ju-Hwan
Kim Ji-Hun
Kim Young-Min
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Abstract
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Cinnamomum camphora is known as a tree that has been used in traditional medicine and food. However, its biological activities in cancer have not yet been clearly investigated. In this study, we investigate the anti-cancer effects of ethanol extract of Cinnamomum camphora on two kinds of human colon cancer cell lines (HCT 116 and HT-29). In previous study, about 50% of cancer cells have p53 mutations. Therefore, two kinds of cancer cells with different p53 gene mutation status are able to verify the effects of cancer more accurately. it is conducted several experiments to prove the effects of anti-cancer through apoptosis and autophagy. It is confirmed the anti-proliferative activity of Cinnamomum camphora ethanol extract through WST-1 assay. Cancer cell migration rate is detected by cell invasion assay and scratched wound healing assay. Also, the expression of apoptosis and autophagy related proteins are assessed by western blot. Our data reveal that Cinnamomum camphora ethanol extract inhibits the cancer cell growth of both p53 gene wild type HCT 116 human colon cancer cells and p53 gene mutation type HT-29 human colon cancer cells. Moreover, it confirms that CCD841 Human colon normal cells are not affected by Cinnamomum camphora ethanol extract. Cinnamomum camphora ethanol extract regulated expression of apoptotic and autophagic proteins in both cancer cells. Then, it is demonstrated that apoptosis and autophagy are induced through the mTOR pathway when treated with rapamycin (mTOR inhibitor). Taken together, these results show that Cinnamomum camphora ethanol extract has considerable potential as chemotherapeutic substance.
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KEYWORD
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Cinnamomum camphora, HCT116 cells, HT-29 cells, apoptosis, autophagy
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